GHK-cU Dosage Calculator: Reconstitution and Units

To calculate GHK-Cu dosage, divide your target dose (mg) by the vial concentration. Concentration = vial mg ÷ BAC water mL. For a 5 mg vial with 2 mL BAC water: concentration is 2.5 mg/mL. A 1 mg dose equals 0.4 mL (40 U-100 units) on a standard insulin syringe.

Example GHK-Cu Dosage Table

Common vial and BAC water combinations with calculated concentration, draw volume, and U-100 syringe units. For education only — verify your vial label before use.

Vial (mg)BAC water (mL)ConcentrationDose (mg)Draw (mL)U-100 units
5 mg2 mL2.5 mg/mL0.5 mg0.2 mL20 units
5 mg2 mL2.5 mg/mL1 mg0.4 mL40 units
5 mg2 mL2.5 mg/mL2 mg0.8 mL80 units
5 mg5 mL1 mg/mL1 mg1 mL100 units
10 mg2 mL5 mg/mL1 mg0.2 mL20 units
10 mg5 mL2 mg/mL1 mg0.5 mL50 units

How to Calculate GHK-Cu Dosage

Three steps convert any vial and BAC water combination into the exact volume to draw on a U-100 insulin syringe.

  1. Step 1 — Calculate concentration (mg/mL)

    Divide the total peptide in the vial (mg) by the volume of BAC water you added (mL). For example: a 5 mg GHK-Cu vial reconstituted with 2 mL BAC water gives 5 ÷ 2 = 2.5 mg/mL.
  2. Step 2 — Calculate draw volume (mL)

    Divide your target dose (mg) by the concentration (mg/mL). For a 0.5 mg dose at 2.5 mg/mL: 0.5 ÷ 2.5 = 0.2 mL. This is the exact volume to draw into the syringe.
  3. Step 3 — Convert to U-100 syringe units

    Multiply the draw volume (mL) by 100. For 0.2 mL: 0.2 × 100 = 20 U-100 units. Read this number on the unit markings of a standard insulin syringe.

Dosage Formula

Concentration formula

Concentration (mg/mL) = Vial mg ÷ BAC water mL

Draw volume formula

Draw volume (mL) = Dose (mg) ÷ Concentration (mg/mL)

U-100 syringe units

U-100 units = Draw volume (mL) × 100

These three formulas apply to any GHK-Cu vial, regardless of size. U-100 is the standard insulin-syringe scale: 100 units = 1 mL.

GHK-Cu Calculator Tool

Enter your vial strength, BAC water volume, and target dose below. The calculator outputs concentration (mg/mL), draw volume (mL), and U-100 units. Educational and research use only.

Example GHK-Cu Calculations

Step-by-step worked examples using common vial sizes. Results are for illustrating the math only.

5 mg vial + 2 mL BAC water, 1 mg dose

  1. Vial: 5 mg  |  BAC water: 2 mL  |  Target dose: 1 mg
  2. Concentration: 5 ÷ 2 = 2.5 mg/mL
  3. Draw volume: 1 ÷ 2.5 = 0.4 mL
  4. U-100 units: 0.4 × 100 = 40 units

5 mg vial + 5 mL BAC water, 1 mg dose (diluted mix — easier to measure)

  1. Vial: 5 mg  |  BAC water: 5 mL  |  Target dose: 1 mg
  2. Concentration: 5 ÷ 5 = 1 mg/mL
  3. Draw volume: 1 ÷ 1 = 1 mL
  4. U-100 units: 1 × 100 = 100 units

10 mg vial + 2 mL BAC water, 2 mg dose

  1. Vial: 10 mg  |  BAC water: 2 mL  |  Target dose: 2 mg
  2. Concentration: 10 ÷ 2 = 5 mg/mL
  3. Draw volume: 2 ÷ 5 = 0.4 mL
  4. U-100 units: 0.4 × 100 = 40 units

GHK-Cu Reconstitution Guide

GHK-Cu is typically supplied as a lyophilised powder in 5 mg or 10 mg vials. Reconstitute with bacteriostatic water (BAC water) using a sterile technique:

  1. Draw the desired BAC water volume into a sterile syringe (1–5 mL is typical for a 5 mg vial).
  2. Inject the BAC water slowly along the vial wall — do not spray directly onto the powder.
  3. Gently swirl (do not shake) until the powder fully dissolves. The solution should be clear.
  4. Store reconstituted GHK-Cu refrigerated (2–8 °C). Most research sources suggest using within 4–6 weeks.

The amount of BAC water you add directly affects concentration. Using more BAC water lowers concentration (larger draw per dose, easier to measure). Using less raises concentration (smaller draw, harder to measure accurately at low doses).

Frequently Asked Questions

How do I calculate the GHK-Cu draw volume?

Divide your target dose (mg) by the concentration (mg/mL). Concentration = vial mg ÷ BAC water mL. For a 5 mg vial with 2 mL BAC: 5 ÷ 2 = 2.5 mg/mL. For a 1 mg dose: 1 ÷ 2.5 = 0.4 mL = 40 U-100 units.

What are U-100 syringe units?

U-100 is the standard scale for insulin syringes. 100 units = 1.0 mL. That means 10 units = 0.1 mL, 50 units = 0.5 mL. Multiply any draw volume in mL by 100 to get the U-100 units reading.

Do I need to recalculate if I change the amount of BAC water?

Yes. Changing BAC water volume changes the concentration (mg/mL), which changes the draw volume for the same mg dose. Always recalculate using: concentration = vial mg ÷ BAC water mL, then draw volume = dose mg ÷ concentration.

What vial sizes does GHK-Cu come in?

GHK-Cu research peptide is commonly available in 5 mg and 10 mg vials. Verify your actual vial label before entering values into the calculator.

Does adding more BAC water change the dose?

No — the total mg in the vial stays the same. Adding more BAC water only lowers concentration, which means you draw a larger volume for the same mg dose. This can make measurement more accurate at small doses.

What is GHK-Cu (copper peptide)?

GHK-Cu (copper tripeptide-1) is a naturally occurring copper-binding peptide studied in skin and tissue research contexts. This calculator provides only the reconstitution and dosage math — it does not provide medical recommendations.

Learn More

Research review and sources

Reviewed by: PeptideUniv Research Team

Updated: March 26, 2026

This page was reviewed against published GHK and GHK-Cu skin and tissue-repair literature plus standard reconstitution math. The dosing examples are measurement examples only, not a treatment protocol.

Primary references and supporting sources

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For educational and research purposes only. Not medical advice. Consult a licensed healthcare professional before making any medical or health-related decisions.