MOTS-c Dosage Calculator: Reconstitution and Units

To calculate MOTS-c dosage, divide your target dose (mg) by the vial concentration. Concentration = vial mg ÷ BAC water mL. For a 5 mg vial with 2 mL BAC water: concentration is 2.5 mg/mL. A 5 mg dose equals 2.0 mL (200 U-100 units) — which would require splitting across two 1 mL syringes.

Example MOTS-c Dosage Table

Common vial and BAC water combinations with calculated concentration, draw volume, and U-100 syringe units. For education only — verify your vial label before use.

Vial (mg)	BAC water (mL)	Concentration	Dose (mg)	Draw (mL)	U-100 units
5 mg	2 mL	2.5 mg/mL	1 mg	0.4 mL	40 units
5 mg	2 mL	2.5 mg/mL	2 mg	0.8 mL	80 units
10 mg	2 mL	5 mg/mL	5 mg	1 mL	100 units
10 mg	5 mL	2 mg/mL	5 mg	2.5 mL	250 units

How to Calculate MOTS-c Dosage

Three steps convert any vial and BAC water combination into the exact volume to draw on a U-100 insulin syringe.

Step 1 — Calculate concentration (mg/mL)
Divide the total peptide in the vial (mg) by the volume of BAC water you added (mL). For example: a 5 mg MOTS-c vial reconstituted with 2 mL BAC water gives 5 ÷ 2 = 2.5 mg/mL.
Step 2 — Calculate draw volume (mL)
Divide your target dose (mg) by the concentration (mg/mL). For a 0.5 mg dose at 2.5 mg/mL: 0.5 ÷ 2.5 = 0.2 mL. This is the exact volume to draw into the syringe.
Step 3 — Convert to U-100 syringe units
Multiply the draw volume (mL) by 100. For 0.2 mL: 0.2 × 100 = 20 U-100 units. Read this number on the unit markings of a standard insulin syringe.

Dosage Formula

Concentration formula

Concentration (mg/mL) = Vial mg ÷ BAC water mL

Draw volume formula

Draw volume (mL) = Dose (mg) ÷ Concentration (mg/mL)

U-100 syringe units

U-100 units = Draw volume (mL) × 100

These three formulas apply to any MOTS-c vial, regardless of size. U-100 is the standard insulin-syringe scale: 100 units = 1 mL.

MOTS-c Calculator Tool

Enter your vial strength, BAC water volume, and target dose below. The calculator outputs concentration (mg/mL), draw volume (mL), and U-100 units. Educational and research use only.

Example MOTS-c Calculations

Step-by-step worked examples using common vial sizes. Results are for illustrating the math only.

5 mg vial + 2 mL BAC water, 2 mg dose

Vial: 5 mg | BAC water: 2 mL | Target dose: 2 mg
Concentration: 5 ÷ 2 = 2.5 mg/mL
Draw volume: 2 ÷ 2.5 = 0.8 mL
U-100 units: 0.8 × 100 = 80 units

10 mg vial + 2 mL BAC water, 5 mg dose

Vial: 10 mg | BAC water: 2 mL | Target dose: 5 mg
Concentration: 10 ÷ 2 = 5 mg/mL
Draw volume: 5 ÷ 5 = 1 mL
U-100 units: 1 × 100 = 100 units

MOTS-c Reconstitution Guide

MOTS-c vials are supplied as lyophilised powder. Reconstitute with BAC water:

Swab both stoppers.
Inject BAC water slowly along the vial wall.
Swirl gently until fully dissolved.
Store at 2–8 °C.

Frequently Asked Questions

How do I calculate MOTS-c draw volume?

Divide your dose (mg) by the concentration. For 5 mg + 2 mL BAC: 2.5 mg/mL. For a 2 mg dose: 2 ÷ 2.5 = 0.8 mL = 80 U-100 units.

What are U-100 syringe units?

U-100 is the standard scale for insulin syringes. 100 units = 1.0 mL. That means 10 units = 0.1 mL, 50 units = 0.5 mL. Multiply any draw volume in mL by 100 to get the U-100 units reading.

Do I need to recalculate if I change the amount of BAC water?

Yes. Changing BAC water volume changes the concentration (mg/mL), which changes the draw volume for the same mg dose. Always recalculate using: concentration = vial mg ÷ BAC water mL, then draw volume = dose mg ÷ concentration.

What is MOTS-c?

MOTS-c is a mitochondria-derived peptide studied in metabolic and energy research. Most evidence is preclinical. This calculator provides only reconstitution math — not dosing recommendations.

Learn More

Research review and sources

Reviewed by: PeptideUniv Research Team

Updated: March 26, 2026

This page was reviewed against the early-stage MOTS-c metabolism literature. Most of the evidence remains preclinical, so the calculator is best used as a measurement tool while the surrounding research picture stays limited.

Primary references and supporting sources

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance — Foundational Cell Metabolism paper that established early obesity and insulin-resistance findings in models.
The mitochondrial-derived peptide MOTS-c is a regulator of plasma metabolites and enhances insulin sensitivity — Human and translational physiology paper often cited for insulin-sensitivity context.

Full Calculator + Protocol Tracker

The PeptideUniv app includes the full reconstitution calculator, dosing schedules, cycle tracker, and reminders — all in one place.

Open full calculator → · Start free trial →

For educational and research purposes only. Not medical advice. Consult a licensed healthcare professional before making any medical or health-related decisions.