SEMAGLUTIDE Dosage Calculator: Reconstitution and Units
To calculate semaglutide dosage, divide your target dose (mg) by the vial concentration. Concentration = vial mg ÷ BAC water mL. For a 5 mg vial with 2 mL BAC water: concentration is 2.5 mg/mL. A 0.5 mg dose equals 0.2 mL (20 U-100 units) on a standard insulin syringe.
Example Semaglutide Dosage Table
Common vial and BAC water combinations with calculated concentration, draw volume, and U-100 syringe units. For education only — verify your vial label before use.
| Vial (mg) | BAC water (mL) | Concentration | Dose (mg) | Draw (mL) | U-100 units |
|---|---|---|---|---|---|
| 5 mg | 2 mL | 2.5 mg/mL | 0.25 mg | 0.1 mL | 10 units |
| 5 mg | 2 mL | 2.5 mg/mL | 0.5 mg | 0.2 mL | 20 units |
| 5 mg | 2 mL | 2.5 mg/mL | 1 mg | 0.4 mL | 40 units |
| 5 mg | 5 mL | 1 mg/mL | 0.5 mg | 0.5 mL | 50 units |
How to Calculate Semaglutide Dosage
Three steps convert any vial and BAC water combination into the exact volume to draw on a U-100 insulin syringe.
Step 1 — Calculate concentration (mg/mL)
Divide the total peptide in the vial (mg) by the volume of BAC water you added (mL). For example: a 5 mg Semaglutide vial reconstituted with 2 mL BAC water gives 5 ÷ 2 = 2.5 mg/mL.Step 2 — Calculate draw volume (mL)
Divide your target dose (mg) by the concentration (mg/mL). For a 0.5 mg dose at 2.5 mg/mL: 0.5 ÷ 2.5 = 0.2 mL. This is the exact volume to draw into the syringe.Step 3 — Convert to U-100 syringe units
Multiply the draw volume (mL) by 100. For 0.2 mL: 0.2 × 100 = 20 U-100 units. Read this number on the unit markings of a standard insulin syringe.
Dosage Formula
Concentration formula
Concentration (mg/mL) = Vial mg ÷ BAC water mLDraw volume formula
Draw volume (mL) = Dose (mg) ÷ Concentration (mg/mL)U-100 syringe units
U-100 units = Draw volume (mL) × 100These three formulas apply to any Semaglutide vial, regardless of size. U-100 is the standard insulin-syringe scale: 100 units = 1 mL.
Semaglutide Calculator Tool
Enter your vial strength, BAC water volume, and target dose below. The calculator outputs concentration (mg/mL), draw volume (mL), and U-100 units. Educational and research use only.
Example Semaglutide Calculations
Step-by-step worked examples using common vial sizes. Results are for illustrating the math only.
5 mg vial + 2 mL BAC water, 0.5 mg dose
- Vial: 5 mg | BAC water: 2 mL | Target dose: 0.5 mg
- Concentration: 5 ÷ 2 = 2.5 mg/mL
- Draw volume: 0.5 ÷ 2.5 = 0.2 mL
- U-100 units: 0.2 × 100 = 20 units
5 mg vial + 2 mL BAC water, 1 mg dose
- Vial: 5 mg | BAC water: 2 mL | Target dose: 1 mg
- Concentration: 5 ÷ 2 = 2.5 mg/mL
- Draw volume: 1 ÷ 2.5 = 0.4 mL
- U-100 units: 0.4 × 100 = 40 units
Semaglutide Reconstitution Guide
Research semaglutide vials are supplied as lyophilised powder. Approved pen injectors (Ozempic, Wegovy) come pre-filled and do not require reconstitution. For research vials:
- Swab both stoppers with alcohol.
- Inject BAC water slowly along the vial wall.
- Swirl gently — do not shake. Solution should be clear.
- Store refrigerated. Avoid repeated freeze-thaw cycles.
Frequently Asked Questions
How do I calculate semaglutide draw volume?
Divide your dose (mg) by the concentration. For 5 mg + 2 mL BAC: 2.5 mg/mL. For 0.5 mg: 0.5 ÷ 2.5 = 0.2 mL = 20 U-100 units.
What are U-100 syringe units?
U-100 is the standard scale for insulin syringes. 100 units = 1.0 mL. That means 10 units = 0.1 mL, 50 units = 0.5 mL. Multiply any draw volume in mL by 100 to get the U-100 units reading.
Do I need to recalculate if I change the amount of BAC water?
Yes. Changing BAC water volume changes the concentration (mg/mL), which changes the draw volume for the same mg dose. Always recalculate using: concentration = vial mg ÷ BAC water mL, then draw volume = dose mg ÷ concentration.
Does the calculator apply to Ozempic or Wegovy pens?
No. Pre-filled pens deliver a fixed dose and do not require reconstitution math. This calculator is for lyophilised research vials that require mixing with BAC water.
Learn More
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Open full calculator → · Start free trial →For educational and research purposes only. Not medical advice. Consult a licensed healthcare professional before making any medical or health-related decisions.